The Big Game XXIX: Bugs vs. Daffy was a 4-hour marathon that aired on Cartoon Network on Saturday, January 27, 2001, from 5:00 PM to 9:00 PM, with the first hour consisting of a pre-game show that featured analysis by experts Jerry Glanville, Nick Buoniconti, Dan Marino, Cris Collinsworth and Len Dawson (for the final time, borrowed from sister property HBO's Inside the NFL) as well as narration by Harry Kalas. The marathon featured 18 Looney Tunes shorts starring Bugs Bunny and Daffy Duck as well as play-by-play and color commentary from Pat Summerall and John Madden during commercials. The marathon was also aired the following day (Super Bowl Sunday) from 1:00 PM to 5:00 PM. There was also a tailgate special of Cartoon Cartoon Fridays on January 26, 2001 preceding the game.
This was the fourth in a series of four Super Bowl spoof marathons that aired annually on Cartoon Network from 1998-2001. These marathons came to be known as "The Big Game"; however, only the final two actually featured this moniker.
For the game itself, clips from the following Bugs-vs.-Daffy cartoon shorts were used:
- Rabbit Fire (most of the first quarter)
- Rabbit Seasoning (end of the first and fourth quarters)
- The Iceman Ducketh (second quarter)
- Duck! Rabbit, Duck! (start of the third quarter)
- The Abominable Snow-Rabbit (end of the third quarter and start of the fourth quarter)
- The Million Hare (most of the fourth quarter)
The game notably ended with a surprise victory for Daffy, with Bugs having thrown the game and left towards the end of the fourth quarter.